Single-stranded DNA-protein binding in the procyclic acidic repetitive protein (PARP) promoter of Trypanosoma brucei
Identifieur interne : 000346 ( France/Analysis ); précédent : 000345; suivant : 000347Single-stranded DNA-protein binding in the procyclic acidic repetitive protein (PARP) promoter of Trypanosoma brucei
Auteurs : Steven Danilo Brown [États-Unis, France] ; Lex H. T. Van Der Ploeg [États-Unis]Source :
- Molecular & Biochemical Parasitology [ 0166-6851 ] ; 1994.
English descriptors
- Teeft :
- Acidic, Assay, Binding conditions, Binding reaction, Binding reactions, Biochemical parasitology, Biol, Boehringer mannheim, Bovine serum albumin, Brucei, Coding strand, Competitor, Competitor designations, Complex formation, Control reaction, Denatured, Final concentration, Fragment, Gene, Largest subunit, Linker, Linker scanning mutagenesis, Locus, Molar, Mutagenesis, Nonspecific competitor, Nonspecific competitors, Nuclear extracts, Nuclear protein, Nucleic, Nucleic acids, Nucleotide, Oligonucleotide, Oligonucleotide probe, Oligonucleotide probes, Oligonucleotides, Parasitology, Parp, Parp genes, Parp promoter, Ploeg, Polymerase, Probe, Probe probe, Procyclic, Procyclic acidic, Procyclic form, Procyclic trypanosomes, Promoter, Proteinase, Restriction fragment, Restriction fragments, Retardation, Retardation analysis, Retardation experiments, Retardation reactions, Rnase, Rrna, Transcription, Trypanosoma, Trypanosoma brucei, Trypanosome, Trypanosomes, Unlabeled, Unlabeled probe, Unlabeled restriction fragment.
Abstract
Abstract: We performed gel retardation analyses of DNA-protein interactions using DNA from the procyclic acidic repetitive protein (PARP) promoter of the protozoan parasite Trypanosoma brucei. The PARP genes of Trypanosoma brucei are transcribed in an α-amanitin resistant manner, and it has been proposed that RNA polymerase I, rather than RNA polymerase II, transcribes the PARP genes [11]. Double-stranded restriction fragments containing the essential PARP-promoter regions bound only sequence-nonspecific nuclear factors, even though protein factors that bind specifically to double-stranded DNA from the snRNA U2 promoter were present in the extracts. In contrast, single-stranded DNA-binding proteins bound with high affinity, nucleotide-sequence and strand-specificity to the −69-−55 element and the coding and non-coding strands of the −37/−11 element.
Url:
DOI: 10.1016/0166-6851(94)90120-1
Affiliations:
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T." last="Van Der Ploeg">Lex H. T. Van Der Ploeg</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Genetics and Molecular Biology, Merck Research Laboratories, Rahway, NJ 07065</wicri:regionArea><wicri:noRegion>NJ 07065</wicri:noRegion></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular & Biochemical Parasitology</title><title level="j" type="abbrev">MOLBIO</title><idno type="ISSN">0166-6851</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">65</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="109">109</biblScope><biblScope unit="page" to="122">122</biblScope></imprint><idno type="ISSN">0166-6851</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0166-6851</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acidic</term><term>Assay</term><term>Binding conditions</term><term>Binding reaction</term><term>Binding reactions</term><term>Biochemical parasitology</term><term>Biol</term><term>Boehringer mannheim</term><term>Bovine serum albumin</term><term>Brucei</term><term>Coding strand</term><term>Competitor</term><term>Competitor designations</term><term>Complex formation</term><term>Control reaction</term><term>Denatured</term><term>Final concentration</term><term>Fragment</term><term>Gene</term><term>Largest subunit</term><term>Linker</term><term>Linker scanning mutagenesis</term><term>Locus</term><term>Molar</term><term>Mutagenesis</term><term>Nonspecific competitor</term><term>Nonspecific competitors</term><term>Nuclear extracts</term><term>Nuclear protein</term><term>Nucleic</term><term>Nucleic acids</term><term>Nucleotide</term><term>Oligonucleotide</term><term>Oligonucleotide probe</term><term>Oligonucleotide probes</term><term>Oligonucleotides</term><term>Parasitology</term><term>Parp</term><term>Parp genes</term><term>Parp promoter</term><term>Ploeg</term><term>Polymerase</term><term>Probe</term><term>Probe probe</term><term>Procyclic</term><term>Procyclic acidic</term><term>Procyclic form</term><term>Procyclic trypanosomes</term><term>Promoter</term><term>Proteinase</term><term>Restriction fragment</term><term>Restriction fragments</term><term>Retardation</term><term>Retardation analysis</term><term>Retardation experiments</term><term>Retardation reactions</term><term>Rnase</term><term>Rrna</term><term>Transcription</term><term>Trypanosoma</term><term>Trypanosoma brucei</term><term>Trypanosome</term><term>Trypanosomes</term><term>Unlabeled</term><term>Unlabeled probe</term><term>Unlabeled restriction fragment</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We performed gel retardation analyses of DNA-protein interactions using DNA from the procyclic acidic repetitive protein (PARP) promoter of the protozoan parasite Trypanosoma brucei. The PARP genes of Trypanosoma brucei are transcribed in an α-amanitin resistant manner, and it has been proposed that RNA polymerase I, rather than RNA polymerase II, transcribes the PARP genes [11]. Double-stranded restriction fragments containing the essential PARP-promoter regions bound only sequence-nonspecific nuclear factors, even though protein factors that bind specifically to double-stranded DNA from the snRNA U2 promoter were present in the extracts. In contrast, single-stranded DNA-binding proteins bound with high affinity, nucleotide-sequence and strand-specificity to the −69-−55 element and the coding and non-coding strands of the −37/−11 element.</div></front></TEI><affiliations><list><country><li>France</li><li>États-Unis</li></country><region><li>État de New York</li><li>Île-de-France</li></region><settlement><li>New York</li><li>Paris</li></settlement><orgName><li>Université Columbia</li></orgName></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Brown, Steven Danilo" sort="Brown, Steven Danilo" uniqKey="Brown S" first="Steven Danilo" last="Brown">Steven Danilo Brown</name></region><name sortKey="Van Der Ploeg, Lex H T" sort="Van Der Ploeg, Lex H T" uniqKey="Van Der Ploeg L" first="Lex H. T." last="Van Der Ploeg">Lex H. T. Van Der Ploeg</name></country><country name="France"><region name="Île-de-France"><name sortKey="Brown, Steven Danilo" sort="Brown, Steven Danilo" uniqKey="Brown S" first="Steven Danilo" last="Brown">Steven Danilo Brown</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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